Physico-Chemical Properties and Biocompatibility of Thermosensitive Chitosan Lactate and Chitosan Chloride Hydrogels Developed for Tissue Engineering Application

Journal of Functional Biomaterials

Ireneusz Majsterek¹  Grzegorz Galita¹  Zofia Modrzejewska²  Katarzyna Pieklarz²  Ewa Kucharska³  Waldemar Maniukiewicz⁴  Michał Tylman⁵ 

[1] Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Narutowicza 60 Street, 90-136 Lodz, Poland;Department of Environmental Engineering, Faculty of Process and Environmental Engineering, Lodz University of Technology, Wolczanska 213 Street, 90-924 Lodz, Poland;Department of Gerontology, Geriatrics and Social Work, Jesuit University Ignatianum in Krakow, Kopernika 26 Street, 31-501 Krakow, Poland;Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116 Street, 90-924 Lodz, Poland;PGE Gornictwo i Energetyka Konwencjonalna S.A., Weglowa 5 Street, 97-400 Belchatow, Poland;

关键词: tissue engineering;    natural polymer;    chitosan;    thermosensitive hydrogel;    structural properties;    cytotoxicity;   

DOI  :  10.3390/jfb12020037

来源: DOAJ

【 摘 要 】

Recently, the modification of the initial structure of biopolymers, mainly chitosan, has been gaining importance with a view to obtain functional forms with increased practicality and specific properties enabling their use in tissue engineering. Therefore, in this article, the properties (structural and biological) of thermosensitive hydrogels obtained from chitosan lactate/chloride and two types of crosslinking agents (β-glycerol phosphate disodium salt pentahydrate and uridine 5′-monophosphate disodium salt) are discussed. The aim of the research is to identify changes in the structure of the biomaterials during conditioning in water. Structural investigations were carried out by FTIR spectroscopy. The crystallinity of gels was determined by X-ray diffraction analysis. The biocompatibility (evaluation of cytotoxicity and genotoxicity) of chitosan hydrogels was investigated by contact with human colon adenocarcinoma cell line for 48 h. The cytotoxicity was verified based on the colorimetric resazurin assay, and the genotoxicity was checked by the comet assay (percentage of DNA in the comet tail). The conducted research showed that the analyzed types of chitosan hydrogels are non-cytotoxic and non-genotoxic materials. The good biocompatibility of chitosan hydrogels surfaces makes them interesting scaffolds with clinical potential in tissue regeneration engineering.

【 授权许可】

Unknown