Transcriptional profiling of genes involved in n-hexadecane compounds assimilation in the hydrocarbon degrading Dietzia cinnamea P4 strain

Brazilian Journal of Microbiology

Luciano Procópio¹  Lucy Seldin¹  Jan Dirk van Elsas²  Michele de Cassia Pereira e Silva² 

[1] Universidade Federal do Rio de Janeiro;University of Groningen;

关键词: Dietzia cinnamea;    n-hexadecane assimilation;    alk genes;    rubredoxin-alkane monooxygenase;    real-time quantitative RT-PCR;   

DOI  :  10.1590/S1517-83822013000200044

来源: DOAJ

【 摘 要 】

The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.

【 授权许可】

Unknown