Candidate Markers of Olaparib Response from Genomic Data Analyses of Human Cancer Cell Lines

Cancers

Anne-Marie Mes-Masson¹  Euridice Carmona¹  Setor Amuzu²  Patricia N. Tonin²  Celia M. T. Greenwood²  Ioannis Ragoussis² 

[1] Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CRCHUM), Montreal, QC H2X 0A9, Canada;Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada;

关键词: olaparib;    genomic markers;    PARP1;    cancer cell lines;   

DOI  :  10.3390/cancers13061296

来源: DOAJ

【 摘 要 】

The benefit of PARP inhibitor olaparib in relapsed and advanced high-grade serous ovarian carcinoma (HGSOC) is well established especially in BRCA1/2 mutation carriers. Identification of additional biomarkers can help expand the population of patients most likely to benefit from olaparib treatment. To identify candidate markers of olaparib response we analyzed genomic and in vitro olaparib response data from two independent groups of cancer cell lines. Using pan-cancer cell lines (n = 896) from the Genomics of Drug Sensitivity in Cancer database, we applied linear regression methods to identify statistically significant gene predictors of olaparib response based on mRNA expression. We then analyzed whole exome sequencing and mRNA gene expression data from our collection of 18 HGSOC cell lines previously classified as sensitive, intermediate, or resistant based on in vitro olaparib response for mutations, copy number variation and differential expression of candidate olaparib response genes. We identify genes previously associated with olaparib response (SLFN11, ABCB1), and discover novel candidate olaparib sensitivity genes with known functions including interaction with PARP1 (PUM3, EEF1A1) and involvement in homologous recombination DNA repair (ELP4). Further investigations at experimental and clinical levels are required to validate novel candidates, and ultimately determine their efficacy as potential biomarkers of olaparib sensitivity.

【 授权许可】

Unknown