Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice

Di-san junyi daxue xuebao

SU Wenting¹  CUI Yu²  LI Xiaoxu²  GUAN Libin² 

[1] Laboratory of Stem Cell and Tissue Engineering & Department of Pathophysiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016;Department of High Altitude Physiology and High Altitude Biology, Key Laboratory of Extreme Environmental Medicine of Ministry of Education, Key Laboratory of High Altitude Medicine of PLA, Faculty of High Altitude Military Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China;

关键词: nitric oxide;    erythrocytes;    hemoglobin;    oxygen affinity;    hypoxia tolerance;   

DOI  :  10.16016/j.1000-5404.201812238

来源: DOAJ

【 摘 要 】

Objective To study the effects of nitric oxide (NO) inhalation on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice. Methods Peripheral blood from 8-week-old male mice was exposed to nitrogen gas containing NO in an oxygenation pool to generate the oxygen dissociation curve and calculate the oxygen affinity parameter P50 and the Hill coefficient. Fifty 8-week-old male C57 mice were randomized into 5 groups (n=10) and subjected to inhalation of 21% O2, 5% O2, or 5% O2 with 5, 10 or 20 mL/m3 NO under anesthesia. The oxygen saturation in the tail vein blood of the mice was measured with an oximeter following the exposures, and the survival time of the mice was recorded. Results The introduction of 100 and 200 mL/m3 NO into the pooled whole blood resulted in a significant left shift of the oxygenation curve, and significantly decreased P50 (P < 0.05) in a concentration-dependent manner. Hypoxic exposure of the mice caused a significant reduction in the oxygen saturation level in the tail vein blood, and this reduction was obviously attenuated by inhalation of 20 mL/m3 NO (P < 0.05). Compared with hypoxia exposure, inhalation of 5, 10, and 20 mL/m3 NO concentration-dependently prolonged the survival time of the mice, and the effect was statistically significantly in mice inhaling 10 and 20 mL/m3 NO (P < 0.05). In mice inhaling 20 mL/m3 NO, the survival time under hypoxic conditions was positively correlated with the level of blood oxygen saturation (P < 0.05). Conclusion Inhalation of NO increases hemoglobin oxygen affinity and improves blood oxygen saturation in mice exposed to hypoxic conditions. NO enhances hypoxia tolerance by improving the oxygen-carrying capacity of the red blood cells.

【 授权许可】

Unknown