Strontium Uptake and Intra-Population 87Sr/86Sr Variability of Bones and Teeth—Controlled Feeding Experiments With Rodents (Rattus norvegicus, Cavia porcellus)

Frontiers in Ecology and Evolution

Théo Tacail¹  Daniela E. Winkler²  Marcus Clauss³  Federico Lugli⁴  Michael Weber⁵  Regina Mertz-Kraus⁵  Thomas Tütken⁵  Katrin Weber⁵  Jennifer Leichliter⁵ 

[1] Bristol Isotope Group, School of Earth Sciences, University of Bristol, Bristol, United Kingdom;Center of Natural History, University of Hamburg, Hamburg, Germany;Clinic for Zoo Animals, Exotic Pets and Wildlife, Vetsuisse Faculty, University of Zürich, Zurich, Switzerland;Department of Cultural Heritage, University of Bologna, Ravenna, Italy;Institute of Geosciences, Johannes Gutenberg University, Mainz, Germany;

关键词: strontium isotopes;    rodents;    population variability;    enamel;    bioavailability;    bone;   

DOI  :  10.3389/fevo.2020.569940

来源: DOAJ

【 摘 要 】

Strontium isotopes in biogenic apatite, especially enamel, are widely employed to determine provenance and track migration in palaeontology and archaeology. Body tissues record the 87Sr/86Sr of bioavailable Sr of ingested food and water. To identify non-local individuals, knowledge of the 87Sr/86Sr of a non-migratory population is required. However, varying factors such as tissue turnover rates, feeding selectivity, Sr content, digestibility of food, and the ingestion of mineral dust can influence body tissue 87Sr/86Sr. To evaluate the Sr contribution of diet and water to mammalian hard tissues 87Sr/86Sr, controlled feeding studies are necessary. Here we present 87Sr/86Sr from controlled feeding experiments with two rodent species (Rattus norvegicus, Cavia porcellus). Due to the continuous and fast incremental growth of rat and guinea pig incisors (~0.1 – 0.5 mm/day), their enamel is expected to record isotopic dietary changes. For Experiment-1: Diet Switch, animals were switched from their respective supplier food to a pelleted experimental diet containing either insect-, plant-, or meat-meal and a staggered-sampling approach was used to monitor the 87Sr/86Sr changes in rat incisor enamel and bone over the course of the experiment. In Experiment-2: Basic Diets, separated cohorts (n = 6) of rats and guinea pigs were fed one of the three pelleted diets and received tap water for 54 days. While the rat incisors showed a complete tissue turnover, the slower-growing guinea pig incisors partially retained supplier diet-related isotopic compositions. In addition, one group of rats fed plant-meal pellets received Sr-rich mineral water, demonstrating that drinking water can be an important Sr source in addition to diet. Additionally, a leaching experiment showed that only a small fraction of diet-related Sr is bioavailable. Finally, in Experiment-3: Dust Addition, guinea pigs were fed pellets with and without addition of 4% of isotopically distinct dust (loess or kaolin). Animals that received kaolin-containing pellets displayed increased enamel 87Sr/86Sr. Intra-population 87Sr/86Sr variability within each feeding group was small and thus we conclude that it should not affect interpretations of 87Sr/86Sr in provenance studies. However, the differences between bulk food and leachate 87Sr/86Sr highlight the importance of Sr bioavailability for provenance studies and Sr isoscapes.

【 授权许可】

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