Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase

Frontiers in Pharmacology

Guang-xin Li¹  Yu-guang Du²  Zhen-yu Li⁴  Xin-hui Fan⁴  Lian-jie Cui⁴  Ai-ping Li⁴  Xing-yun Zhao⁴  Xue-qin Li⁴  Yue-tao Liu⁴  Xue-mei Qin⁴  Ke Li⁴ 

[1] College of Agriculture, Shanxi Agricultural University, Taiyuan, China;Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China;Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China;Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China;The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China;

关键词: APS-Ⅱ;    enzymolysis;    structure analysis;    immune activity;    structure-activity relationship;   

DOI  :  10.3389/fphar.2022.839635

来源: DOAJ

【 摘 要 】

Astragali Radix polysaccharides (APSs) have a wide range of biological activities. Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs. However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification. In this study, APS-II was degraded by endo α-1,4-glucosidase. The degraded products with different degrees of polymerization [1–3 (P1), 3–6 (P2), 7–14 (P3), and 10–18 (P4)] were obtained using a polyacrylamide gel chromatography column. The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC–MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis. Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products. The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches. Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components. In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.

【 授权许可】

Unknown