AMB Express | |
MicroRNA-143 targets MAPK3 to regulate the proliferation and bone metastasis of human breast cancer cells | |
Mingzhu Huang1  Zhiqiang Wu1  Yanchun Meng1  Wangjun Yan1  Yiqun Du1  Jian Zhang1  | |
[1] Department of Oncology, Shanghai Medical College, Fudan University; | |
关键词: Breast cancer; Metastasis; Cell proliferation; Migration; Invasion; Chemo-sensitivity; | |
DOI : 10.1186/s13568-020-01072-w | |
来源: DOAJ |
【 摘 要 】
Abstract MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissues and cell lines. The miR-143 has inhibitory effect on CAMA-1cell growth which was manifested as significant (P < 0.05) decline in loss of viability of cancer cells. The loss of cell viability was revealed to be due to the induction of apoptotic cell death as evident from acridine orange/ethidium bromide (AO/EB) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. The apoptotic cell percentage was found to be 35.7% in miR-143 mimics transfected in comparison to 6.4% in miR-NC transfected cells. The western blot analysis showed that miR-143 caused enhancement in Bax and suppression in Bcl-2 expression in CAMA-1 cells. The miR-143 also suppressed the bone metastasis of the CAMA-1 cells by suppressing the expression of Jag1 and deactivation of the Rho-signalling pathway. The transwell assays also showed considerable anti-metastatic effects of miR-143 on CAMA-1 cells. Taken together, miR-143 has growth inhibitory anti-metastatic effect on breast cancer and thus may prove beneficial in breast cancer treatment.
【 授权许可】
Unknown