MicroRNA-143 targets MAPK3 to regulate the proliferation and bone metastasis of human breast cancer cells

AMB Express

Mingzhu Huang¹  Zhiqiang Wu¹  Yanchun Meng¹  Wangjun Yan¹  Yiqun Du¹  Jian Zhang¹ 

[1] Department of Oncology, Shanghai Medical College, Fudan University;

关键词: Breast cancer;    Metastasis;    Cell proliferation;    Migration;    Invasion;    Chemo-sensitivity;   

DOI  :  10.1186/s13568-020-01072-w

来源: DOAJ

【 摘 要 】

Abstract MicroRNAs (miRs) have shown tremendous potential to act as therapeutic targets for cancer treatment. In this context, the present study was designed to investigate the potential of miR-143 in the treatment of breast cancer. Results showed that miR-143 to be significantly (P < 0.05) downregulated in breast cancer tissues and cell lines. The miR-143 has inhibitory effect on CAMA-1cell growth which was manifested as significant (P < 0.05) decline in loss of viability of cancer cells. The loss of cell viability was revealed to be due to the induction of apoptotic cell death as evident from acridine orange/ethidium bromide (AO/EB) and 4′,6-diamidino-2-phenylindole (DAPI) staining assays. The apoptotic cell percentage was found to be 35.7% in miR-143 mimics transfected in comparison to 6.4% in miR-NC transfected cells. The western blot analysis showed that miR-143 caused enhancement in Bax and suppression in Bcl-2 expression in CAMA-1 cells. The miR-143 also suppressed the bone metastasis of the CAMA-1 cells by suppressing the expression of Jag1 and deactivation of the Rho-signalling pathway. The transwell assays also showed considerable anti-metastatic effects of miR-143 on CAMA-1 cells. Taken together, miR-143 has growth inhibitory anti-metastatic effect on breast cancer and thus may prove beneficial in breast cancer treatment.

【 授权许可】

Unknown