Electrical Stimulation through Conductive Substrate to Enhance Osteo-Differentiation of Human Dental Pulp-Derived Stem Cells

Applied Sciences

Wei-Wen Hu¹  Ting-Ling Yen²  Chien-Hsun Chen³  Yu-Che Cheng⁴  Hong-Wei Kuo⁴  Ya-Yuan Mao⁴ 

[1] Department of Chemical and Materials Engineering, National Central University, Zhongli District, Taoyuan City 32001, Taiwan;Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan;Department of Periodontics, Cathay General Hospital, Taipei 10630, Taiwan;Proteomics Laboratory, Department of Medical Research, Cathay General Hospital, Taipei 10630, Taiwan;

关键词: human dental pulp stem cells;    substrate-mediated electrical stimulation;    direct current electric field;    osteo-differentiation;    bone morphogenesis proteins;   

DOI  :  10.3390/app9183938

来源: DOAJ

【 摘 要 】

Human dental pulp-derived stem cells (hDPSCs) are promising cellular sources for bone healing. The acceleration of their differentiation should be beneficial to their clinical application. Therefore, a conductive polypyrrole (PPy)-made electrical stimulation (ES) device was fabricated to provide direct-current electric field (DCEF) treatment, and its effect on osteo-differentiation of hDPSCs was investigated in this study. To determine the optimal treating time, electrical field of 0.33 V/cm was applied to hDPSCs once for 4 h on different days after the osteo-induction. The alizarin red S staining results suggested that ES accelerated the mineralization rates of hDPSCs. The quantification analysis results revealed a nearly threefold enhancement in calcium deposition by ES at day 0, 2, and 4, whereas the promotion effect in later stages was in vain. To determine the ES-mediated signaling pathway, the expression of genes in the bone morphogenetic protein (BMP) family and related receptors were quantified using qPCR. In the early stages of osteo-differentiation, the mRNA levels of BMP2, BMP3, BMP4, and BMP5 were increased significantly in the ES groups, indicating that these genes were involved in the specific signaling routes induced by ES. We are the first using DCEF to improve the osteo-differentiation of hDPSCs, and our results promise the therapeutic applications of hDPSCs on cell-based bone tissue engineering.

【 授权许可】

Unknown