Molecular cloning and in-depth bioinformatics analysis of type II ribosome-inactivating protein isolated from Sambucus ebulus

Saudi Journal of Biological Sciences

Ali Dehestani¹  Ali Pakdin-Parizi²  Masoumeh Rezaei-Moshaei³  Ali Bandehagh³  Majid Golkar⁴ 

[1] Corresponding authors at: Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran (A. Pakdin-Parizi);Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran (A. Bandehagh).;Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz, Iran;Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran;

关键词: Physicochemical properties;    Protein modeling;    Recombinant RIP;   

DOI  :  

来源: DOAJ

【 摘 要 】

Plant ribosome–inactivating proteins (RIPs) are N–glycosidases which inhibit protein synthesis through depurination of the ribosomal RNA sequence. Type II RIPs are heterodimer proteins which can bind to cell surfaces. The cytotoxicity of these RIPs is different. Sambucus spp. are a rich source of RIP proteins with different properties. In the present study, a type II RIP was isolated from S. ebulus plant that grows widely in the north of Iran, and different bioinformatics tools were used for the evaluation of physicochemical, functional and 3D protein characteristics. The results showed significant differences among isolated RIP and other Sambucus RIP proteins. The study of these differences can not only expand our insight into the functioning mechanisms of plant RIPs but also provide information about a novel RIP protein with potential biological applications.

【 授权许可】

Unknown