Advances in Azorella glabra Wedd. Extract Research: In Vitro Antioxidant Activity, Antiproliferative Effects on Acute Myeloid Leukemia Cells and Bioactive Compound Characterization

Molecules

Dilip K. Rai¹  Immacolata Faraone²  Luigi Milella²  Maria Francesca Armentano²  Daniela Russo²  Pellegrino Musto³  Stefania Trino⁴  Daniela Lamorte⁴  Ilaria Laurenzana⁴  Alessandro Sgambato⁵  Antonella Caivano⁶  LucianaDe De Luca⁶ 

[1] Department of Food BioSciences, Teagasc Food Research Centre Ashtown, Dublin D15KN3K, Ireland;Department of Science, University of Basilicata, V.le dell’Ateneo Lucano 10, 85100 Potenza, Italy;Hematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, Italy;Laboratory of Preclinical and Translational Research, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, Italy;Scientific Direction, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, Italy;Unit of Clinical Pathology, Centro di Riferimento Oncologico della Basilicata (IRCCS CROB), 85028 Rionero in Vulture, Potenza, Italy;

关键词: Azorella glabra Wedd.;    Azorella diapensioides A. Gray;    yareta;    phytochemicals;    terpenoids;    antioxidant;   

DOI  :  10.3390/molecules25214890

来源: DOAJ

【 摘 要 】

Azorella glabra Wedd. (AG) is traditionally used to treat gonorrhea or kidney’s problems. The antioxidant, antidiabetic, anticholinesterase and in vitro antitumor activities of AG extracts were recently reported. The aim of this work was to investigate anti-leukemic properties of AG chloroform fraction (AG CHCl₃) and of its ten sub-fractions (I-X) and to identify their possible bioactive compounds. We determined their in vitro antioxidant activity using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), nitric oxide (NO) and superoxide anion (SO) assays, and their phytochemical profile by spectrophotometric and LC-MS/MS techniques. I-X action on two acute myeloid leukemia (AML) cell lines viability, apoptosis and cell cycle were evaluated by MTS, western blotting and cytofluorimetric assays. Different polyphenol, flavonoid and terpenoid amount, and antioxidant activity were found among all samples. Most of I-X induced a dose/time dependent reduction of cell viability higher than parent extract. IV and VI sub-fractions showed highest cytotoxic activity and, of note, a negligible reduction of healthy cell viability. They activated intrinsic apoptotic pathway, induced a G₀/G₁ block in leukemic cells and, interestingly, led to apoptosis in patient AML cells. These activities could be due to mulinic acid or azorellane terpenoids and their derivatives, tentatively identified in both IV and VI. In conclusion, our data suggest AG plant as a source of potential anti-AML agents.

【 授权许可】

Unknown