期刊论文详细信息
eLife
Previously undetected super-spreading of Mycobacterium tuberculosis revealed by deep sequencing
Jean-François Proulx1  Fiona McIntosh2  Robyn S Lee3  Marcel A Behr4  William P Hanage5 
[1] Center for Communicable Disease Dynamics, Harvard TH Chan School of Public Health, Boston, United States;Department of Epidemiology, Harvard TH Chan School of Public Health, Boston, United States;Epidemiology Division, Dalla Lana School of Public Health, University of Toronto, Toronto, Canada;Nunavik Regional Board of Health and Social Services, Kuujjuaq, Canada;The Research Institute of McGill University Health Centre, Montréal, Canada;
关键词: Tuberculosis;    genomic epidemiology;    within-host diversity;    transmission;    outbreaks;   
DOI  :  10.7554/eLife.53245
来源: DOAJ
【 摘 要 】

Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether ‘deep’ sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011–2012, 50 cases). Samples were sequenced to ~500–1000x and reads were aligned to a novel local reference genome generated with PacBio SMRT sequencing. Consensus and heterogeneous variants were identified and compared across genomes. In contrast with previous genomic analyses using ~50x depth, deep sequencing allowed us to identify a novel super-spreader who likely transmitted to up to 17 other cases during the outbreak (35% of the remaining cases that year). It is increasingly evident that within-host diversity should be incorporated into transmission analyses; deep sequencing may facilitate more accurate detection of super-spreaders and transmission clusters. This has implications not only for TB, but all genomic studies of transmission - regardless of pathogen.

【 授权许可】

Unknown   

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