The Enigma of Centriole Loss in the 1182-4 Cell Line

Cells

Chunfeng Zheng¹  TimothyL. Megraw¹  Xiuwen Liu²  Alain Debec³  Benjamin Loppin⁴ 

[1] Department of Biomedical Sciences, College of Medicine, Florida State University, Tallahassee, FL 32306-4300, USA;Department of Computer Science, Florida State University, Tallahassee, FL 32306-4530, USA;Institute of Ecology and Environmental Sciences, iEES, Sorbonne University, UPEC, CNRS, IRD, INRA, F-75005 Paris, France;Laboratoire de Biologie et de Modélisation de la Cellule—CNRS UMR 5239, École Normale Supérieure de Lyon, University of Lyon, F-69007 Lyon, France;

关键词: centriole;    centrosome;    maternal haploid;    Drosophila melanogaster;    cell line;    haploid cells;   

DOI  :  10.3390/cells9051300

来源: DOAJ

【 摘 要 】

The Drosophila melanogaster cell line 1182-4, which constitutively lacks centrioles, was established many years ago from haploid embryos laid by females homozygous for the maternal haploid (mh) mutation. This was the first clear example of animal cells regularly dividing in the absence of this organelle. However, the cause of the acentriolar nature of the 1182-4 cell line remained unclear and could not be clearly assigned to a particular genetic event. Here, we detail historically the longstanding mystery of the lack of centrioles in this Drosophila cell line. Recent advances, such as the characterization of the mh gene and the genomic analysis of 1182-4 cells, allow now a better understanding of the physiology of these cells. By combining these new data, we propose three reasonable hypotheses of the genesis of this remarkable phenotype.

【 授权许可】

Unknown