Long Noncoding RNA LINC00460 Promotes Hepatocellular Carcinoma Progression via Regulation of miR-342-3p/AGR2 Axis

OncoTargets and Therapy

关键词: hepatocellular carcinoma;    lncrna;    linc00460;    mir-342-3p;    agr2;    migration;    invasion.;   

DOI  :  

来源: DOAJ

【 摘 要 】

Jing Yang,1,2 Kun Li,3 Jian Chen,4 Xiaoxiong Hu,5,6 He Wang,2 Xuan Zhu1 1Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 2Department of Gastroenterology and Hepatology, The People’s Hospital of Yichun City, Yichun 336000, People’s Republic of China; 3Department of General Surgery, The People’s Hospital of Yichun City, Yichun 336000, People’s Republic of China; 4Department of Oncology, The People’s Hospital of Yichun City, Yichun 336000, People’s Republic of China; 5Clinic Research Center of People’s Hospital of Yichun City, Yichun 336000, People’s Republic of China; 6Department of Infection Disease, The People’s Hospital of Yichun City, Yichun 336000, People’s Republic of ChinaCorrespondence: Xuan ZhuDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Road, Donghu District, Nanchang 330006, Jiangxi, People’s Republic of ChinaTel [phone]Email [e-mail]: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death worldwide. LINC00460, a novel long non-coding RNA (lncRNA), was recently confirmed as an oncogene in various cancers. However, the biological function and underlying mechanism of LINC00460 in HCC is largely obscure.Methods: Fifty pairs of tumor tissue and adjacent normal tissues from HCC patients, as well as six HCC cell lines and a normal human hepatic epithelial cell line were subjected to qRT-PCR assay to evaluate the expression levels of LINC00460. CCK-8 assays were used to detect the proliferation of HCC cells. Transwell assay was used to measure the migration and invasion abilities of HCC cells. RNA pull-down and luciferase assays were performed to verify the direct interaction between LINC00460 and miR-342-3p. A xenograft model of HCC was established to validate the in vivo function of LINC00460 in HCC progression.Results: We firstly detected LINC00460 expression was significantly upregulated in both HCC tumor tissues and cell lines. The upregulation of LINC00460 was positively associated with HCC progression. Functionally, LINC00460 facilitated HCC cell proliferation, migration, and invasion capacities, which due to that LINC00460 could physically bind to and repress miR-342-3p to elevate the expression of AGR2.Conclusion: Our data firstly reveal the clinical relevance, biological function, and regulatory mechanism of LINC00460 in HCC development. LINC00460 promotes HCC progression by elevating AGR2 expression via sponging miR-342-3p, providing a promising therapeutic target for HCC treatment.Keywords: hepatocellular carcinoma, LncRNA, LINC00460, miR-342-3p, AGR2, migration, invasion

【 授权许可】

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