Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning

eLife

Tadatsune Iida¹  Shinji Urata²  Masamichi Yamamoto²  Yu Mizushima³  Yu Matsumoto⁴  Tatsuya Yamasoba⁴  Shigeo Okabe⁴  Chisato Fujimoto⁴ 

[1] Department of Otolaryngology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, Japan;Department of Otolaryngology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;

关键词: organ of Corti;    optical tissue clearing;    hair cell;    hearing loss;    hard tissue;    machine learning;   

DOI  :  10.7554/eLife.40946

来源: DOAJ

【 摘 要 】

The highly organized spatial arrangement of sensory hair cells in the organ of Corti is essential for inner ear function. Here, we report a new analytical pipeline, based on optical clearing of tissue, for the construction of a single-cell resolution map of the organ of Corti. A sorbitol-based optical clearing method enabled imaging of the entire cochlea at subcellular resolution. High-fidelity detection and analysis of all hair cell positions along the entire longitudinal axis of the organ of Corti were performed automatically by machine learning–based pattern recognition. Application of this method to samples from young, adult, and noise-exposed mice extracted essential information regarding cellular pathology, including longitudinal and radial spatial characteristics of cell loss, implying that multiple mechanisms underlie clustered cell loss. Our method of cellular mapping is effective for system-level phenotyping of the organ of Corti under both physiological and pathological conditions.

【 授权许可】

Unknown