Enhanced Oil Removal by a Non-Toxic Biosurfactant Formulation

Energies

Raquel Diniz Rufino¹  Hugo Moraes Meira²  Juliana Moura de Luna²  Emília Mendes da Silva Santos²  Leonie Asfora Sarubbo²  Darne Germano de Almeida²  IsabelaRegina Alvares da Silva Lira²  Jaciana dos Santos Aguiar³  Attilio Converti⁴  AlessandroAlberto Casazza⁴ 

[1] Advanced Institute of Technology and Innovation (IATI), Rua Joaquim de Brito, 216, Boa Vista, Recife 50070-280, Brazil;Biotechnology Sector, Catholic University of Pernambuco (UNICAP), Rua do Príncipe, n. 526, Boa Vista, Recife 50050-900, Brazil;Center of Biological Sciences, Department of Antibiotics, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, Cidade Universitária, CEP, Recife 54740-520, Brazil;Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Università degli Studi di Genova (UNIGE), Via Opera Pia 15, I-16145 Genova, Italy;

关键词: biosurfactant;    industrial waste;    petroleum contamination;    Candida;   

DOI  :  10.3390/en14020467

来源: DOAJ

【 摘 要 】

In this study, a new formulation of low-cost, biodegradable, and non-toxic biosurfactant by Candida sphaerica UCP 0995 was investigated. The study was conducted in a bioreactor on an industrial waste-based medium, and a central composite rotatable design was used for optimization. The best results, namely a 25.22 mN/m reduction in surface tension, a biosurfactant yield of 10.0 g/L, and a critical micelle concentration of 0.2 g/L, were achieved in 132 h at an agitation speed of 175 rpm and an aeration rate of 1.5 vvm. Compositional and spectroscopic analyses of the purified biosurfactant by chemical methods, Fourier transform infrared spectroscopy, and nuclear magnetic resonance suggested that it is a glycolipid-type biosurfactant, and it showed no cytotoxicity in the MTT assay. The biosurfactant, submitted to different formulation methods as a commercial additive, remained stable for 120 days at room temperature. Tensioactive properties and stability were evaluated at different pH values, temperatures, and salt concentrations. The biosurfactant obtained with all formulation methods demonstrated good stability, with tolerance to wide ranges of pH, temperature and salinity, enabling application under extreme environmental conditions. Bioremediation tests were performed to check the efficacy of the isolated biosurfactant and the selected microbial species in removing oil from soil. The results demonstrated that the biosurfactant produced has promising properties as an agent for the bioremediation of contaminated soil.

【 授权许可】

Unknown