Effect of rutin on oxidative and proinflammatory damage induced by cisplatin in blood serum, ureter, bladder and urethra in rats

Biotechnology & Biotechnological Equipment

Gul Mehmet Ali¹  Suleyman Zeynep²  Suleyman Halis³  Erdogan Abdullah⁴  Hirik Erkan⁴  Bozkurt Aliseydi⁴  Ercument Keskin⁴ 

[1] Department of Biochemistry, Faculty of Medicine, Ataturk University;Faculty of Health Sciences, Department of Internal Medicine Nursing, Erzincan Binali Yildirim University;Faculty of Medicine, Department of Pharmacology, Erzincan Binali Yildirim University;Faculty of Medicine, Department of Urology, Erzincan Binali Yildirim University;

关键词: cisplatin;    rutin;    blood serum;    ureter;    urethra;    bladder;   

DOI  :  10.1080/13102818.2020.1727362

来源: DOAJ

【 摘 要 】

Rutin (Vitamin P1) is an antioxidant and anti-inflammatory flavonoid. This study examined the effect of rutin on the potential of cisplatin to cause oxidative and proinflammatory damage in blood serum, ureter, bladder and urethra in rats. Animals were divided into four groups: (i) healthy (HG), (ii) 50 mg/kg of rutin + 5 mg/kg of cisplatin (C + R50), (iii) 100 mg/kg of rutin + 5 mg/kg of cisplatin (C + R100) and (iv) only cisplatin (CG). Rutin and distilled water were applied once a day for eight days. Cisplatin was injected intraperitoneally at a dose of 5 mg/kg for eight days, once every two days. Finally, animals were sacrificed by high -dose anesthesia after blood samples were taken. Ureter, bladder and urethra tissues were removed and malondialdehyde (MDA), myeloperoxidase (MPO), total glutathione (tGSH), superoxide dismutase (SOD) interleukin beta (IL-1β) and tumor necrosis factor alpha (TNF-α) levels were measured. The results showed that the levels of MDA, MPO, IL-1β and TNF-α in the blood serum of the CG group increased significantly (p < 0.0001) and the tGSH and SOD levels decreased compared to the HG. Rutin administration reversed the effect as seen in C + R50 and C + R100 groups. However, there was no statistically significant difference in the levels of MDA, MPO, tGSH, SOD, IL-1β and TNF-α in ureter, bladder and urethra tissue (p > 0.05).This suggests that cisplatin produces oxidative stress in the blood serum but not in the ureter, bladder and urethra tissues. It was observed that rutin prevented the cisplatin-related oxidative and proinflammatory damage in the blood serum.

【 授权许可】

Unknown