A Nanoplasmonic-Based Biosensing Approach for Wide-Range and Highly Sensitive Detection of Chemicals

Nanomaterials

Stefania Di Ronza¹  Francesco Arcadio¹  Aldo Minardo¹  Caterina Eramo¹  Chiara Perri¹  Luigi Zeni¹  Nunzio Cennamo¹  Guido Chiaretti²  Giovanni Porto²  Girolamo D’Agostino² 

[1] Department of Engineering, University of Campania Luigi Vanvitelli, Via Roma, 29, 81031 Aversa, Italy;Moresense Srl., Filarete Foundation, Viale Ortles 22/4, 20139 Milan, Italy;

关键词: nanoplasmonic sensors;    slab waveguides;    plastic optical fibers;    biochemical sensors;    optical sensors;    e-beam lithography;   

DOI  :  10.3390/nano11081961

来源: DOAJ

【 摘 要 】

In a specific biosensing application, a nanoplasmonic sensor chip has been tested by an experimental setup based on an aluminum holder and two plastic optical fibers used to illuminate and collect the transmitted light. The studied plasmonic probe is based on gold nanograting, realized on the top of a Poly(methyl methacrylate) (PMMA) chip. The PMMA substrate could be considered as a transparent substrate and, in such a way, it has been already used in previous work. Alternatively, here it is regarded as a slab waveguide. In particular, we have deposited upon the slab surface, covered with a nanograting, a synthetic receptor specific for bovine serum albumin (BSA), to test the proposed biosensing approach. Exploiting this different experimental configuration, we have determined how the orientation of the nanostripes forming the grating pattern, with respect to the direction of the input light (longitudinal or orthogonal), influences the biosensing performances. For example, the best limit of detection (LOD) in the BSA detection that has been obtained is equal to 23 pM. Specifically, the longitudinal configuration is characterized by two observable plasmonic phenomena, each sensitive to a different BSA concentration range, ranging from pM to µM. This aspect plays a key role in several biochemical sensing applications, where a wide working range is required.

【 授权许可】

Unknown