Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium

Frontiers in Microbiology

Hiroko eIijima¹  Takashi eOsanai¹  Akihiko eKondo²  Yuka eNakaya²  Masami Yokota Hirai²  Mami eOkamoto²  Tomokazu eShirai² 

[1] Meiji University;RIKEN;

关键词: Cyanobacteria;    Metabolism;    Metabolomics;    Sigma Factor;    succinate;   

DOI  :  10.3389/fmicb.2015.01064

来源: DOAJ

【 摘 要 】

Succinate is a building block compound that the U.S. Department of Energy has declared as important in biorefineries, and it is widely used as a commodity chemical. Here, we identified the two genes increasing succinate production of the unicellular cyanobacterium Synechocystis sp. PCC 6803. Succinate was excreted under dark, anaerobic conditions, and its production level increased by knocking out ackA, which encodes an acetate kinase, and by overexpressing sigE, which encodes an RNA polymerase sigma factor. Glycogen catabolism and organic acid biosynthesis were enhanced in the mutant lacking ackA and overexpressing sigE, leading to an increase in succinate production reaching 5 times of the wild-type levels. Our genetic and metabolomic analyses thus demonstrated the effect of genetic manipulation of a metabolic enzyme and a transcriptional regulator on succinate excretion from this cyanobacterium with the data based on metabolomic technique.

【 授权许可】

Unknown