Electrochemical Glucose Quantification as a Strategy for Ethanolic Fermentation Monitoring

Chemosensors

Flavio Della Pelle¹  Jorge Hoyos-Arbeláez²  Isabel Acevedo-Restrepo²  MarioV. Vázquez²  Lucas Blandón-Naranjo² 

[1] Faculty of Biosciences and Technologies for Food, Agriculture and Environment, University of Teramo, 64023 Teramo, Italy;Grupo Interdisciplinario de Estudios Moleculares-GIEM, Instituto de Química, Universidad de Antioquia, Calle 67 No. 53–108, Medellín A.A 1226, Colombia;

关键词: fermentation;    NADH;    glucose;    cyclic voltammetry;    chronoamperometry;    electrochemical sensor;    biosensor;   

DOI  :  10.3390/chemosensors7010014

来源: DOAJ

【 摘 要 】

The possibility of using an electrochemical biosensor, using screen-printed electrodes modified with a carbonaceous material and a commercial kit for the determination of glucose, to monitor an ethanolic fermentation was analyzed. The determination is based on the electrochemical oxidation reaction of NADH that occurs at a potential where the components of the kit do not generate a current signal, even in the presence of the fermentation medium. The electrochemical system was used to analyze the variation of glucose concentration during a laboratory-scale fermentation. The results were contrasted with the variation of standard characterization parameters such as pH, total soluble solids (TSS), the viability of the yeast, and concentration of ethanol produced. Of these values, the total soluble solids should be related to the concentration of glucose obtained by the electrochemical sensor, however, this last measure is more specific for sugar since the TSS refers to all soluble solids. The obtained results allow us to verify the usefulness of the electrochemical method for real-time monitoring of a fermentation.

【 授权许可】

Unknown