Extracellular Vesicles of Human Periodontal Ligament Stem Cells Contain MicroRNAs Associated to Proto-Oncogenes: Implications in Cytokinesis

Frontiers in Genetics

Jacopo Pizzicannella¹  Guya Diletta Marconi²  Oriana Trubiani²  Emanuela Mazzon³  Serena Silvestro³  Luigi Chiricosta³  Placido Bramanti³  Agnese Gugliandolo³ 

[1] ASL02 Lanciano-Vasto-Chieti, “Ss. Annunziata” Hospital, Chieti, Italy;Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti and Pescara, Chieti, Italy;IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina, Italy;

关键词: human periodontal ligament stem cells;    extracellular vesicles;    next generation sequencing;    non-coding RNAs;    microRNAs;    cytokinesis;   

DOI  :  10.3389/fgene.2020.00582

来源: DOAJ

【 摘 要 】

The human Periodontal Ligament Stem Cells (hPDLSCs) exhibit self-renewal capacity and clonogenicity potential. The Extracellular Vesicles (EVs) secreted by hPDLSCs are particles containing lipids, proteins, mRNAs, and non-coding RNAs, among which microRNAs, that are important in intercellular communication. The purpose of this study was the analysis of the non-coding RNAs contained in the EVs derived from hPDLSCs using Next Generation Sequencing. Moreover, our data were enriched using bioinformatic tools. The analysis highlighted the presence of non-coding RNAs and five microRNAs: MIR24-2, MIR142, MIR335, MIR490, and MIR296. Our results show that these miRNAs target the genes classified in two terms of the Gene Ontology: “Ras protein signal transduction” and “Actin/microtubule cytoskeleton organization.” Noteworthy, the in-deep analysis of our EVs highlights that the miRNAs could be implicated in the silencing of proto-oncogenes involved in 12 different types of tumors.

【 授权许可】

Unknown