Frontiers in Genetics | |
Extracellular Vesicles of Human Periodontal Ligament Stem Cells Contain MicroRNAs Associated to Proto-Oncogenes: Implications in Cytokinesis | |
Jacopo Pizzicannella1  Guya Diletta Marconi2  Oriana Trubiani2  Emanuela Mazzon3  Serena Silvestro3  Luigi Chiricosta3  Placido Bramanti3  Agnese Gugliandolo3  | |
[1] ASL02 Lanciano-Vasto-Chieti, “Ss. Annunziata” Hospital, Chieti, Italy;Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti and Pescara, Chieti, Italy;IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina, Italy; | |
关键词: human periodontal ligament stem cells; extracellular vesicles; next generation sequencing; non-coding RNAs; microRNAs; cytokinesis; | |
DOI : 10.3389/fgene.2020.00582 | |
来源: DOAJ |
【 摘 要 】
The human Periodontal Ligament Stem Cells (hPDLSCs) exhibit self-renewal capacity and clonogenicity potential. The Extracellular Vesicles (EVs) secreted by hPDLSCs are particles containing lipids, proteins, mRNAs, and non-coding RNAs, among which microRNAs, that are important in intercellular communication. The purpose of this study was the analysis of the non-coding RNAs contained in the EVs derived from hPDLSCs using Next Generation Sequencing. Moreover, our data were enriched using bioinformatic tools. The analysis highlighted the presence of non-coding RNAs and five microRNAs: MIR24-2, MIR142, MIR335, MIR490, and MIR296. Our results show that these miRNAs target the genes classified in two terms of the Gene Ontology: “Ras protein signal transduction” and “Actin/microtubule cytoskeleton organization.” Noteworthy, the in-deep analysis of our EVs highlights that the miRNAs could be implicated in the silencing of proto-oncogenes involved in 12 different types of tumors.
【 授权许可】
Unknown