Mid-pregnancy maternal immune activation increases Pax6-positive and Tbr2-positive neural progenitor cells and causes integrated stress response in the fetal brain in a mouse model of maternal viral infection

IBRO Neuroscience Reports

Hiroki Shimada¹  Hiromi Sakata-Haga²  Tsuyoshi Tsukada³  Hiroki Shoji³  Toshihisa Hatta⁴ 

[1] Corresponding author at: Department of Anatomy, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan.;Department of Neurosurgery, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan;Department of Anatomy, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan;Department of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan;

关键词: Maternal immune activation;    Polyriboinosinic–polyribocytidylic acid;    Integrated stress response;    Activating transcription factor 4;    Unfolded protein response;    Neurogenesis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Maternal immune activation (MIA) in midpregnancy is a risk factor for neurodevelopmental disorders. Improper brain development may cause malformations of the brain; maldevelopment induced by MIA may lead to a pathology-related phenotype. In this study, a single intraperitoneal injection of 20 mg/kg polyriboinosinic–polyribocytidylic acid [poly(I:C)] was administered to C57BL/6J mice on embryonic day (E) 12.5 to mimic maternal viral infection. Histopathological analysis of neurogenesis was performed using markers for Pax6, Tbr2, and Tbr1. In these fetuses, significant increases were observed in the proportion of Pax6-positive neural progenitor cells and Pax6/Tbr2 double-positive cells 24 h after poly(I:C) injection. There were no differences in the proportion of Tbr1-positive postmitotic neurons 48 h after poly(I:C) injection. At E18.5, there were more Pax6-positive and Tbr2-positive neural progenitor cells in the poly(I:C)-injected group than in the saline-injected group. Gene ontology enrichment analysis of poly(I:C)-induced differentially expressed genes in the fetal brain at E12.5 demonstrated that these genes were enriched in terms including response to cytokine, response to decreased oxygen levels in the category of biological process. At E13.5, activating transcription factor 4 (Atf4), which is an effector of integrated stress response, was significantly upregulated in the fetal brain. Our results show that poly(I:C)-induced MIA at E12.5 leads to dysregulated neurogenesis and upregulates Atf4 in the fetal brain. These findings provide a new insight in the mechanism of MIA causing improper brain development and subsequent neurodevelopmental disorders.

【 授权许可】

Unknown