Si-Ni-San Prevents Reserpine-Induced Depression by Inhibiting Inflammation and Regulating CYP450 Enzymatic Activity

Frontiers in Pharmacology

Yang Zong¹  Hongli Dong²  Ting Chen²  Lijing Zhu³  Wenzheng Ju³ 

[1] Clinical Pharmacy Laboratory of TCM, Academy of Wumen Chinese Medicine, Suzhou, China;Clinical Pharmacy Laboratory of TCM, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China;Department of Clinical Pharmacology Laboratory, Affiliated Hospital to Nanjing University of Chinese Medicine, Nanjing, China;

关键词: depression;    reserpine;    Si-Ni-San;    anti-inflammation;    CYP450 enzymes;   

DOI  :  10.3389/fphar.2019.01518

来源: DOAJ

【 摘 要 】

Depression is becoming a major public health concern worldwide. Si-Ni-San (SNS) is a famous formula in Traditional Chinese Medicine (TCM) with potent antidepressant effects. However, the antidepressant mechanism of SNS has not been clearly elucidated. This study was performed to verify whether the antidepressant effects of SNS were related to its anti-inflammatory effects, the levels of brain-derived neurotrophic factor (BDNF) and Cytochrome P450 (CYP450) enzymatic activity. In our study, behavioral tests such as the forced swim test, sucrose preference test and open-field test were evaluated to ensure the establishment of depressive rats. The levels of IL-1β, IL-6, and TNF-α in the serum, liver, and hippocampus of rats were measured by enzyme-linked immunosorbent assays (ELISA). Furthermore, the key proteins NF-κB, BDNF, and TrkB were analyzed by Western blot (WB) analysis in the hippocampus. In addition, CYP450 enzymatic activity analysis was performed using LC-MS/MS in conjunction with drug and statistics (DAS 3.0) after oral administration of six probe drugs. Our results showed that SNS attenuated reserpine-induced increases in IL-1β, IL-6, and TNF-α expression in the serum, liver, and hippocampus. The levels of NF-κB, BDNF, and TrkB in the hippocampus of depressive rats were also altered. According to the pharmacokinetic parameters, SNS had moderate inhibitory effects in the reserpine-induced depression model on CYP1A2, CYP2D1, CYP2E1, and CYP3A2, but no significant metabolic changes to CYP2C6 and CYP2D2. These findings suggested that SNS has a protective effect on reserpine-induced depressive rats, which may be related to the improvement of the inflammatory factors, the level of BDNF and the activity of CYP450 enzymes.

【 授权许可】

Unknown