Aging, Bone Marrow and Next-Generation Sequencing (NGS): Recent Advances and Future Perspectives

International Journal of Molecular Sciences

Payal Ganguly¹  Shelly Pathak²  Bradley Toghill² 

[1] Leeds Institute of Rheumatic and Musculoskeletal Medicine, University of Leeds, Leeds LS9 7TF, UK;Novogene Europe, 25 Science Park, Milton, Cambridge CB4 0FW, UK;

关键词: aging;    bone marrow;    stem cells;    next-generation sequencing (NGS);    genomics;    inflammaging;   

DOI  :  10.3390/ijms222212225

来源: DOAJ

【 摘 要 】

The aging of bone marrow (BM) remains a very imperative and alluring subject, with an ever-increasing interest among fellow scientists. A considerable amount of progress has been made in this field with the established ‘hallmarks of aging’ and continued efforts to investigate the age-related changes observed within the BM. Inflammaging is considered as a low-grade state of inflammation associated with aging, and whilst the possible mechanisms by which aging occurs are now largely understood, the processes leading to the underlying changes within aged BM remain elusive. The ability to identify these changes and detect such alterations at the genetic level are key to broadening the knowledgebase of aging BM. Next-generation sequencing (NGS) is an important molecular-level application presenting the ability to not only determine genomic base changes but provide transcriptional profiling (RNA-seq), as well as a high-throughput analysis of DNA–protein interactions (ChIP-seq). Utilising NGS to explore the genetic alterations occurring over the aging process within alterative cell types facilitates the comprehension of the molecular and cellular changes influencing the dynamics of aging BM. Thus, this review prospects the current landscape of BM aging and explores how NGS technology is currently being applied within this ever-expanding field of research.

【 授权许可】

Unknown