Cancers | |
Distinct 3D Structural Patterns of Lamin A/C Expression in Hodgkin and Reed-Sternberg Cells | |
Roberta Vanni1  Fabio Contu2  Landon Wark2  Ludger Klewes2  Aline Rangel-Pozzo2  Peter Trokajlo2  Hans Knecht3  JohnG. Gartner4  NathalieA. Johnson5  Sabine Mai5  Tina Petrogiannis-Haliotis5  Yuval Garini6  | |
[1] Institute of Nanotechnology, Bar Ilan University, Ramat Gan 5290002, Israel;Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada;Department of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, 09042 Cagliari, Italy;Department of Pathology, University of Manitoba, Winnipeg, MB R3E 0W2, Canada;Division of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montréal, QC H3G 2M1, Canada;;Physics Department & | |
关键词: Hodgkin’s Lymphoma; Reed-Sternberg cells; Lamin A/C patterns; 3D imaging; Lymphocytes; nuclear architecture; | |
DOI : 10.3390/cancers10090286 | |
来源: DOAJ |
【 摘 要 】
Classical Hodgkin’s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients’ samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A/C protein could be a useful tool for understanding nuclear remodeling in cHL.
【 授权许可】
Unknown