Validation of exercise-response genes in skeletal muscle cells of Thoroughbred racing horses

Animal Bioscience

Hak Kyo Lee¹  Hyo Gun Lee²  Byung-Wook Cho²  Nipin Sp³  Young Mok Yang³  Dong Young Kang³  Kyoung-Jin Jang³  Doh Hoon Kim³ 

[1] Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea;Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea;Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, Korea;

关键词: thoroughbred;    exercise stress;    skeletal muscle cells;    gene expression;   

DOI  :  10.5713/ajas.18.0749

来源: DOAJ

【 摘 要 】

Objective To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.

【 授权许可】

Unknown