Chemical Profile and Evaluation of the Antioxidant and Anti-Acetylcholinesterase Activities of Annona squamosa L. (Annonaceae) Extracts

Foods

Selene Maia de Morais¹  José Galberto Martins da Costa¹  Débora Odília Duarte Leite¹  Natália Kelly Gomes de Carvalho²  Gerson Javier Torres Salazar²  Cicera Janaine Camilo²  Carla de Fatima Alves Nonato² 

[1] Northeast Biotechnology Network, Postgraduate Program in Biotechnology, State University of Ceará, Fortaleza 60740-000, CE, Brazil;Research Laboratory of Natural Products, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-000, CE, Brazil;

关键词: Annona squamosa;    antioxidant;    acetylcholinesterase;    chemical prospection;   

DOI  :  10.3390/foods10102343

来源: DOAJ

【 摘 要 】

This study presents the chemical profile of extracts from the pulp and seed of Annona squamosa L., as well as the evaluation of their antioxidant and acetylcholinesterase inhibition activities. In the chemical prospection, qualitative assays were performed, and the contents of total phenols, flavonoids, vitamin C, and carotenoids were quantified. For the compounds identification, analyses of the extracts were performed by liquid chromatography coupled to mass spectrometry. Antioxidant evaluation was performed using the DPPH, ABTS, Fe³⁺ reduction, 2-DR protection, and β-carotene protection methods. The assay for inhibition of acetylcholinesterase activity was determined using the method described by Ellman. The secondary metabolites identified were anthocyanidins, flavones, flavonols, and alkaloids. Phenol analysis showed a higher quantitative value of total phenols and flavonoids for the seed extract, and the vitamin C content was higher in the pulp extract. There was no significant difference in relation to the carotenoids quantification. The best results obtained for antioxidant activity, for both seed and pulp extracts, were with the ABTS method with IC₅₀ of 0.14 ± 0.02 and 0.38 ± 0.02 mg/mL, respectively. Compared to A. squamosa seed extract, the pulp extract demonstrates higher AChE inhibitory activity with IC₅₀ of 18.82 ± 0.17 µg/mL. A. squamosa is a nutritious food source. The continuity of the studies is fundamental to relate the consumption of this food and its effects on neurodegenerative diseases.

【 授权许可】

Unknown