Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagy

Stem Cell Research & Therapy

Xueping Li¹  Kai Cheng¹  Mingxia Gao¹  Xinwei Wang¹  Xiaoju Wang¹  Qiang Lin¹  Peng Xia¹  Tingting Zhang¹ 

[1] Department of Rehabilitation Medicine, Nanjing First Hospital, Nanjing Medical University;

关键词: Low-intensity pulsed ultrasound (LIPUS);    Mesenchymal stem cells (MSCs);    Chondrogenesis;    Autophagy;   

DOI  :  10.1186/s13287-019-1142-z

来源: DOAJ

【 摘 要 】

Abstract Background Low-intensity pulsed ultrasound (LIPUS) can induce mesenchymal stem cell (MSC) differentiation, although the mechanism of its potential effects on chondrogenic differentiation is unknown. Since autophagy is known to regulate the differentiation of MSCs, the aim of our study was to determine whether LIPUS induced chondrogenesis via autophagy regulation. Methods MSCs were isolated from the rat bone marrow, cultured in either standard or chondrogenic medium, and stimulated with 3 MHz of LIPUS given in 20% on–off cycles, with or without prior addition of an autophagy inhibitor or agonist. Chondrogenesis was evaluated on the basis of aggrecan (AGG) organization and the amount of type II collagen (COL2) and the mRNA expression of AGG, COL2, and SRY-related high mobility group-box gene 9 (SOX9) genes. Results LIPUS promoted the chondrogenic differentiation of MSCs, as shown by the changes in the extracellular matrix (ECM) proteins and upregulation of chondrogenic genes, and these effects were respectively augmented and inhibited by the autophagy inhibitor and agonist. Conclusions Taken together, these results indicate that LIPUS promotes MSC chondrogenesis by inhibiting autophagy.

【 授权许可】

Unknown