Decontamination of Titanium Surface Using Different Methods: An In Vitro Study

Materials

Kosuke Kashiwagi¹  Takayoshi Kawazoe¹  Tetsuji Kusumoto²  Liji Chen³  Naoyuki Matsumoto³  Yuichiro Tashiro⁴  Seiji Takao⁴  Honghao Zhang⁴  Sifan Yan⁴  Satoshi Komasa⁴  Yuhao Zeng⁴  Min Li⁴  Joji Okazaki⁴  Yuanyuan Yang⁴  Akinori Agariguchi⁴  Yasuyuki Kobayashi⁵ 

[1] Department of Fixed Prosthodontics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan;Department of Oral Health Engineering, Faculty of Health Sciences, Osaka Dental University, Osaka 573-1121, Japan;Department of Orthodntics, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan;Department of Removable Prosthodontics and Occlusion, Osaka Dental University, 8-1 Kuzuha-hanazono-cho, Hirakata, Osaka 573-1121, Japan;Osaka Research Institute of Industrial Science and Technology Morinomiya Center, 1-6-50, Morinomiya, Joto-ku, Osaka-shi 536-8553, Japan;

关键词: Ti implant;    hydrocarbon decontamination;    Finevo cleaning system;    ultraviolet treatment;    plasma;   

DOI  :  10.3390/ma13102287

来源: DOAJ

【 摘 要 】

Contamination of implants is inevitable during different steps of production as well as during the clinical use. We devised a new implant cleaning strategy to restore the bioactivities on dental implant surfaces. We evaluated the efficiency of the Finevo cleaning system, and Ultraviolet and Plasma treatments to decontaminate hydrocarbon-contaminated titanium disks. The surfaces of the contaminated titanium disks cleaned using the Finevo cleaning system were similar to those of the uncontaminated titanium disks in scanning electron microscopy and X-ray photoelectron spectroscopy analysis, but no obvious change in the roughness was observed in the scanning probe microscopy analysis. The rat bone marrow mesenchymal stem cells (rBMMSCs) cultured on the treated titanium disks attached to and covered the surfaces of disks cleaned with the Finevo cleaning system. The alkaline phosphatase activity, calcium deposition, and osteogenesis-related gene expression in rBMMSCs on disks cleaned using the Finevo cleaning system were higher compared to those in the ultraviolet and plasma treatments, displaying better cell functionality. Thus, the Finevo cleaning system can enhance the attachment, differentiation, and mineralization of rBMMSCs on treated titanium disk surfaces. This research provides a new strategy for cleaning the surface of contaminated titanium dental implants and for restoration of their biological functions.

【 授权许可】

Unknown