Changes in Nuclear Shape and Gene Expression in Response to Simulated Microgravity Are LINC Complex-Dependent

International Journal of Molecular Sciences

Ceasar Udave¹  Richard Barker²  Simon Gilroy²  Brian Richardson³  Mark Cameron⁴  HowardG. Levine⁵  Srujana Neelam⁵  Ye Zhang⁵ 

[1] Department of Biomedical Engineering, Arizona State University, Tempe, AZ 85281, USA;Department of Botany, University of Wisconsin-Madison, Madison, WI 53706, USA;Department of Nutrition, Case Western Reserve University, Cleveland, OH 44106, USA;Universities Space Research Association, Columbia, MD 21046, USA;Utilization and Life Sciences, NASA John F. Kennedy Space Center, Kennedy Space Center, Merritt Island, FL 32899, USA;

关键词: LINC complex;    simulated microgravity;    nuclear morphology;   

DOI  :  10.3390/ijms21186762

来源: DOAJ

【 摘 要 】

Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. In our study, we used a simulated microgravity device, a 3-D clinostat (Gravite), to investigate whether the LINC complex mediates cellular responses to the simulated microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.

【 授权许可】

Unknown