Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes

Micromachines

Ayelen Tayagui¹  Yiling Sun¹  Sarah Sale¹  Debolina Sarkar¹  Volker Nock¹  Ashley Garrill¹ 

[1] Biomolecular Interaction Centre, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8041, New Zealand;

关键词: fungi;    oomycetes;    lab-on-a-chip;    high-throughput screening;    force measurement;   

DOI  :  10.3390/mi12060639

来源: DOAJ

【 摘 要 】

Pathogenic fungi and oomycetes give rise to a significant number of animal and plant diseases. While the spread of these pathogenic microorganisms is increasing globally, emerging resistance to antifungal drugs is making associated diseases more difficult to treat. High-throughput screening (HTS) and new developments in lab-on-a-chip (LOC) platforms promise to aid the discovery of urgently required new control strategies and anti-fungal/oomycete drugs. In this review, we summarize existing HTS and emergent LOC approaches in the context of infection strategies and invasive growth exhibited by these microorganisms. To aid this, we introduce key biological aspects and review existing HTS platforms based on both conventional and LOC techniques. We then provide an in-depth discussion of more specialized LOC platforms for force measurements on hyphae and to study electro- and chemotaxis in spores, approaches which have the potential to aid the discovery of alternative drug targets on future HTS platforms. Finally, we conclude with a brief discussion of the technical developments required to improve the uptake of these platforms into the general laboratory environment.

【 授权许可】

Unknown