PP2C – like promoter and its deletion variants are induced by ABA but not by MeJA and SA in Arabidopsis thaliana

Frontiers in Plant Science

Rajesh eMehrotra¹  Purva eBhalotia¹  Sandhya eMehrotra¹  Chetna eSangwan¹  Anshu eAlok² 

[1] Birla Institute of Technology and Science;National Agri-Food Biotechnology Institute (NABI);

关键词: Abscisic Acid;    abiotic stress;    ACGT cis element;    Cis regulatory elements;    Gene regulation.;   

DOI  :  10.3389/fpls.2016.00547

来源: DOAJ

【 摘 要 】

Gene expression is mediated through interaction between cis regulatory elements and its cognate transcription factors. Cis regulatory elements are defined as non-coding DNA sequences that provide the binding sites for transcription factors and are clustered in the upstream region of genes. ACGT cis regulatory elementis one of the important cis regulatory elements found to be involved in diverse biological processes likeauxin response, salicylic acid response, UV light response, ABA response and jasmonic acid response .We identified through in silicoanalysis that the upstream region of protein phosphatase 2C (PP2C)gene has a distinct genetic architecture of ACGT elements.In the present study, the activation ofthe full length promoter and its deletion constructs like 900 base pair, 500 base pair, 400 base pair and NRMwere examined by stable transformation in Arabidopsis thaliana using β glucuronidase as the reporter gene. Evaluation of deletion constructs ofPP2C- like promoter was carried out in the presence of phytohormones like abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA). Our result indicatedthat the full length and 900 base pair promoter-reporter constructs of PP2C-like promoter was induced in response to ABA but not to methyl jasmonate and salicylic acid.

【 授权许可】

Unknown