How to speed up the polymerase chain reaction

Biomolecular Detection and Quantification

关键词: Real-time PCR;    Taq polymerase;    Denaturation;    Annealing;    Polymerisation;   

DOI  :  10.1016/j.bdq.2017.05.002

来源: DOAJ

【 摘 要 】

Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

【 授权许可】

Unknown