Progress towards sugar beet improvement through somatic hybridization I. Inactivation of nuclei and cytoplasm in donor and recipient protoplasts

Acta Societatis Botanicorum Poloniae

Elżbieta Jażdżewska¹  Anna Majewska-Sawka¹  Aleksandra Niklas¹ 

[1]Plant Breeding and Acclimatization Institute

关键词: Beta vulgaris L.;    suspension cultures;    protoplasts;    asymmetric protoplasts;   

DOI  :  10.5586/asbp.1995.044

来源: DOAJ

【 摘 要 】

The isolation and culture of suspension-derived protoplasts from two sugar beet (Beta vulgaris L.) genotypes are described. Immobilization of protoplasts in agarose resulted in high frequency divisions and microcallus regeneration, with plating efficiency (PE) being clearly genotype-dependent. In further studies towards asymmetric fusion experiments, the effect of different doses of ultraviolet radiation (UV) and iodoacetic acid (IA) on protoplast physiology was assessed. Viability of both treated (UV, IA) and untreated protoplasts (control) was determined by FDA staining, and the biological effect was evaluated by testing the ability of protoplasts to divide and to form calli. The results are discussed in terms of the applicability of the methods for the production of asymmetric protoplasts suitable for somatic hybridization within the genus Beta.

【 授权许可】

Unknown