Production and Characterization of a Novel Biosurfactant Molecule from Bacillus safensis YKS2 and Assessment of Its Efficiencies in Wastewater Treatment by a Directed Metagenomic Approach

Sustainability

Chithravel Vadivalagan¹  Naif Abdullah Al-Dhabi²  Mariadhas Valan Arasu²  Srinivasan Venkatesan³  Balasubramanian Balamuralikrishnan⁴  Rasiravathanahalli Kaveriyappan Govindarajan⁵  Dharman Kalaimurugan⁵  Chartchai Khanongnuch⁵  Shreeshivadasan Chelliapan⁶  Hesam Kamyab⁷ 

[1] Department of Biochemistry, School of Medicine, AKFA University, Tashkent 100042, Uzbekistan;Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;Department of Environmental Science, School of Life Sciences, Periyar University, Salem 636 011, India;Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, Korea;Division of Biotechnology, Faculty of Agro-Industry, School of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand;Engineering Department, Razak Faculty of Technology & Informatics, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, Kuala Lumpur 54100, Malaysia;Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, Kuala Lumpur 54100, Malaysia;

关键词: biosurfactant;    biodegradation;    metagenomics;    glycolipid;    wastewater treatment;    macromolecule;   

DOI  :  10.3390/su14042142

来源: DOAJ

【 摘 要 】

Biosurfactant is a biodegradation accelerator that improves bioavailability and facilitates degradation by microorganisms. The study was meant to produce a novel biosurfactant molecule from Bacillussafensis YKS2. An efficient biosurfactant-producing strain, namely, Bacillus safensis YKS2, was selected using hemolytic activity, drop collapsing test, oil spreading test and blue agar plate methods in four oil-degrading strains isolated from a soil sample. Biosurfactant production in the optimization of bacteria culture conditions by RSM is a statistical grouping technique that is analyzed using the AVOVA approach to surface tention. In addition, the study was characterized by UV spectrophotometer FT-IR, HR-SEM, and GC-MS analyses to explain its structural and chemical details. Wastewater treatment was monitored for pH, EC, turbidity, alkalinity, chemical oxygen demand (COD), biochemical oxygen demand (BOD) and dissolved oxygen (DO) in order to justify the efficacy of the biosurfactant during wastewater treatment. The results of the UV spectrophotometer showed absorption at 530 nm, and the FT-IR analyzed carboxylic acids, alcohol and phenols groups, whichthe GC-MS analysis indicated were lipopeptide purified by hexadecanoic andtetradecanoic processes, respectively. The results show that the wastewater removal efficiency of 70% wasachieved within 24 h. In comparison, metagenomics was conducted during the treatment process to identify changes in the microbial load and diversity, which essentially indicatethe biosurfactant performance of the wastewater treatment process. The microbial load in the treated biosurfactant wastewater (84,374 sequences) was greatly decreased compared to untreated wastewater (139,568 sequences). It was concluded that B. safensis YKS2, producing a glycolipid form of biosurfactant, has possible benefits in the remediation of wastewater, and can be used for large-scale processing inbiosurfactant industries.

【 授权许可】

Unknown