Virome of respiratory secretion from children with unknown etiological acute respiratory disease revealed recombinant human parechovirus and other significant viruses

Virology Journal

Hao Wang¹  Ying Liu²  Guang-Ming Sun²  Jie Yang³  Jian Zeng³ 

[1] Department of Clinical Laboratory, The Affiliated Huai’an Hospital of Xuzhou Medical University;Department of Clinical Laboratory, Xuzhou Central Hospital;School of Medicine, Jiangsu University;

关键词: Respiratory secretion;    Viral metagenomics;    Parechovirus;    Anellovirus;    Coxsackievirus;   

DOI  :  10.1186/s12985-021-01586-0

来源: DOAJ

【 摘 要 】

Abstract Using viral metagenomics, viral nucleic acid in 30 respiratory secretion samples collected from children with unknown etiological acute respiratory disease were investigated. Sequences showing similarity to human parainfluenza virus 1, anellovirus, bocavirus, coxsackievirus A4, human parechovirus (HPeV), and alphaflexivirus were recovered from these samples. Complete genomes of one anellovirus, one coxsackievirus A4, three parechoviruses were determined from these libraries. The anellovirus (MW267851) phylogenetically clustered with an unpublished anellovirus (MK212032) from respiratory sample of a Vietnamese patient, forming a separate branch neighboring to strains within the genus Betatorquevirus. The genome of coxsackievirus A4 (MW267852) shares the highest sequence identity of 96.4% to a coxsackievirus A4 (MN964079) which was identified in clinical samples from children with Hand, Foot, and Mouth Disease (HFMD). Two (MW267853 and MW267854) of the three parechoviruses belong to HPeV-1 and the other one (MW267855) belongs to HPeV-6. Recombination analysis indicated that an HPeV-1 (MW267854) identified in this study is a putative recombinant occurred between HPeV-1 and HPeV-3. Whether these viruses have association with specific respiratory disease calls for further investigation.

【 授权许可】

Unknown