Advanced Methods for Detection of Bacillus cereus and Its Pathogenic Factors

Sensors

Seav-Ly Tran¹  Nalini Ramarao¹  Marco Marin¹  Jasmina Vidic¹ 

[1] INRAE, AgroParisTech, Micalis Institute, Université Paris‐Saclay, 78350 Jouy‐en‐Josas, France;

关键词: Bacillus cereus;    bacterial detection;    biosensors;    toxin detection;    food safety;    human health;   

DOI  :  10.3390/s20092667

来源: DOAJ

【 摘 要 】

Bacillus cereus is an opportunistic foodborne pathogen causing food intoxication and infectious diseases. Different toxins and pathogenic factors are responsible for diarrheal syndrome, like nonhemolytic enterotoxin Nhe, hemolytic enterotoxin Hbl, enterotoxin FM and cytotoxin K, while emetic syndrome is caused by the depsipeptide cereulide toxin. The traditional method of B. cereus detection is based on the bacterial culturing onto selective agars and cells enumeration. In addition, molecular and chemical methods are proposed for toxin gene profiling, toxin quantification and strain screening for defined virulence factors. Finally, some advanced biosensors such as phage-based, cell-based, immunosensors and DNA biosensors have been elaborated to enable affordable, sensitive, user-friendly and rapid detection of specific B. cereus strains. This review intends to both illustrate the state of the B. cereus diagnostic field and to highlight additional research that is still at the development level.

【 授权许可】

Unknown