Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Biosensors

Kyeong-Hwan Lee¹  Mahaveer D. Kurkuri²  Venkatachalam Thendral²  Madhuprasad Kigga²  Mahesh Padmalaya Bhat²  Krishna Kant³  Tariq Altalhi⁴  Uluvangada Thammaiah Uthappa⁵ 

[1] Agricultural Automation Research Center, Chonnam National University, Gwangju 61186, Korea;Centre for Research in Functional Materials (CRFM), Jain Global Campus, Jain University, Bengaluru 562112, Karnataka, India;Departamento de Química Física, Campus Universitario, CINBIO Universidade de Vigo, 36310 Vigo, Spain;Department of Chemistry, Faculty of Science, Taif University, Taif 21944, Saudi Arabia;School of Chemical Engineering, Yeungnam University, 280 Daehak-ro, Gyeongsan 38541, Korea;

关键词: circulating tumor cells (CTCs);    microfluidic device;    physical method;    biological method;    cancer diagnostics;   

DOI  :  10.3390/bios12040220

来源: DOAJ

【 摘 要 】

CTCs (circulating tumor cells) are well-known for their use in clinical trials for tumor diagnosis. Capturing and isolating these CTCs from whole blood samples has enormous benefits in cancer diagnosis and treatment. In general, various approaches are being used to separate malignant cells, including immunomagnets, macroscale filters, centrifuges, dielectrophoresis, and immunological approaches. These procedures, on the other hand, are time-consuming and necessitate multiple high-level operational protocols. In addition, considering their low efficiency and throughput, the processes of capturing and isolating CTCs face tremendous challenges. Meanwhile, recent advances in microfluidic devices promise unprecedented advantages for capturing and isolating CTCs with greater efficiency, sensitivity, selectivity and accuracy. In this regard, this review article focuses primarily on the various fabrication methodologies involved in microfluidic devices and techniques specifically used to capture and isolate CTCs using various physical and biological methods as well as their conceptual ideas, advantages and disadvantages.

【 授权许可】

Unknown