Role of heme Oxygenase-1 in low dose Radioadaptive response

Redox Biology

Tom K. Hei¹  Jie Ma²  Wei Han²  Jue Hou²  Lingzhi Bao²  Guodong Chen²  K.N. Yu³ 

[1] Center for Radiological Research, Columbia University, New York, NY, USA;Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, China;Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Hong Kong;

关键词: Heme Oxygenase 1;    Radioadaptive response;    Hemin;    Znpp;    Nrf2;    Reactive oxidative species;   

DOI  :  10.1016/j.redox.2016.03.002

来源: DOAJ

【 摘 要 】

Radioadaptive response (RAR) is an important phenomenon induced by low dose radiation. However, the molecular mechanism of RAR is obscure. In this study, we focused on the possible role of heme oxygenase 1 (HO-1) in RAR. Consistent with previous studies, priming dose of X-ray radiation (1–10 cGy) induced significant RAR in normal human skin fibroblasts (AG 1522 cells). Transcription and translation of HO-1 was up-regulated more than two fold by a priming dose of radiation (5 cGy). Zinc protoporphyrin Ⅸ, a specific competitive inhibitor of HO-1, efficiently inhibited RAR whereas hemin, an inducer of HO-1, could mimic priming dose of X-rays to induce RAR. Knocking down of HO-1 by transfection of HO-1 siRNA significantly attenuated RAR. Furthermore, the expression of HO-1 gene was modulated by the nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which translocated from cytoplasm to nucleus after priming dose radiation and enhance the antioxidant level of cells.

【 授权许可】

Unknown