Non-Covalent Loading of Anti-Cancer Doxorubicin by Modularizable Peptide Self-Assemblies for a Nanoscale Drug Carrier

Molecules

Makoto Miyatani¹  Shunsuke Kataoka¹  Takuya Ikeda¹  Kohei Kishioka¹  Kin-ya Tomizaki¹  Takahito Imai¹  Mami Komada²  Kenji Usui² 

[1] Department of Materials Chemistry, Ryukoku University, 1-5 Yokotani, Seta-Oe, Otsu 520-2194, Japan;Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minami, Chuo, Kobe 650-0047, Japan;

关键词: peptide self-assembly;    drug delivery system;    cell-penetrating peptide;    nuclear-localization signal;    anti-cancer doxorubicin;   

DOI  :  10.3390/molecules22111916

来源: DOAJ

【 摘 要 】

We prepared nanoscale, modularizable, self-assembled peptide nanoarchitectures with diameters less of than 20 nm by combining β-sheet-forming peptides tethering a cell-penetrating peptide or a nuclear localization signal sequence. We also found that doxorubicin (Dox), an anti-cancer drug, was non-covalently accommodated by the assemblies at a ratio of one Dox molecule per ten peptides. The Dox-loaded peptide assemblies facilitated cellular uptake and subsequent nuclear localization in HeLa cells, and induced cell death even at low Dox concentrations. This peptide nanocarrier motif is a promising platform for a biocompatible drug delivery system by altering the targeting head groups of the carrier peptides.

【 授权许可】

Unknown