Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-Inflammatory Potential in Callus Cultures of Silybum marianum (L.) Gaertn.

Molecules

BilalHaider Abbasi¹  Muhammad Younas¹  Muzamil Shah¹  MuhammadAsad Ullah¹  Nathalie Giglioli-Guivarc’h²  Duangjai Tungmunnithum³  Christophe Hano³  Samantha Drouet³ 

[1] Department of Biotechnology, Quaid-i-Azam University, Islamabad-45320, Pakistan;EA2106 Biomolecules et Biotechnologies Vegetales, Universite Francois-Rabelais de Tours, 37000 Tours, France;Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRA USC1328, Université d’Orléans, 45067 Orléans CEDEX 2, France;

关键词: Silybum marianum (L.) Gaertn.;    light regimes;    melatonin;    antioxidant;    phenolics;    flavonoids;    silymarin;    anti-inflammatory;   

DOI  :  10.3390/molecules24071207

来源: DOAJ

【 摘 要 】

Silybum marianum (L.) Gaertn. is a well-known medicinal herb, primarily used in liver protection. Light strongly affects several physiological processes along with secondary metabolites biosynthesis in plants. Herein, S. marianum was exploited for in vitro potential under different light regimes in the presence of melatonin. The optimal callogenic response occurred in the combination of 1.0 mg/L α-naphthalene acetic acid and 0.5 mg/L 6-benzylaminopurine under photoperiod. Continuous light associated with melatonin treatment increased total flavonoid content (TFC), total phenolic content (TPC) and antioxidant potential, followed by photoperiod and dark treatments. The increased level of melatonin has a synergistic effect on biomass accumulation under continuous light and photoperiod, while an adverse effect was observed under dark conditions. More detailed phytochemical analysis showed maximum total silymarin content (11.92 mg/g dry weight (DW)) when placed under continuous light + 1.0 mg/L melatonin. Individually, the level of silybins (A and B), silydianin, isolsilychristin and silychristin was found highest under continuous light. Anti-inflammatory activities were also studied and highest percent inhibition was recorded against 15-lipoxygenase (15-LOX) for cultures cultivated under continuous light (42.33%). The current study helps us to better understand the influence of melatonin and different light regimes on silymarin production as well as antioxidant and anti-inflammatory activities in S. marianum callus extracts.

【 授权许可】

Unknown