Isolation and identification of new compound, 2,7″-phloroglucinol-6,6′-bieckol from brown algae, Ecklonia cava and its antioxidant effect

Journal of Functional Foods

Sung-Myung Kang¹  Seung-Hong Lee¹  You-Jin Jeon¹  Kil-Nam Kim²  Soo-Jin Heo³ 

[1] Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea;Jeju Biodiversity Research Institute (JBRI) and Jeju Hi-Tech Industry Development Institute (HiDI), Jeju 697-943, Republic of Korea;Marine Living Resources Research Department, Korea Ocean Research and Development Institute, Ansan 426-744, Republic of Korea;

关键词: Ecklonia cava;    2,7″-Phloroglucinol-6,6′-bieckol;    Antioxidant;   

DOI  :  

来源: DOAJ

【 摘 要 】

Brown algae, Ecklonia cava has been regarded as a useful material in the prevention or treatment of several human diseases in Jeju Island, Korea. In this study, we investigated its antioxidant activity, and isolated a new antioxidative compound, which was identified as 2,7″-phloroglucinol-6,6′-bieckol (PHB) on the basis of LC/MS, as well as 1H and 13C NMR spectroscopy results. The antioxidant activity of PHB was evaluated via several in vitro assays, such as radical scavenging activities using electron spin resonance spectrometry, intracellular reactive oxygen species scavenging activity, and DNA damage assays. PHB evidenced profound scavenging activities on DPPH, alkyl, hydroxyl, and superoxide radicals (IC50: 0.51, 2.07, 75.64 and 57.19 μM for DPPH, alkyl, hydroxyl and superoxide radicals, respectively). The antioxidant activity of PHB was higher than that of ascorbic acid. Furthermore, PHB effectively inhibited H2O2-induced DNA damage. The results of this study indicate that PHB may prove useful as a novel natural marine antioxidant.

【 授权许可】

Unknown