Chylomicron metabolism in an animal model for hyperlipoproteinemia type I

Journal of Lipid Research

Magnus Hultin¹  Yan Shen¹  Anna Lindberg¹  Thomas Olivecrona¹  Roger Savonen¹  Björn Christophersen²  Knut Nordstoga³  Gunilla Olivecrona⁴ 

[1] Department of Medical Biosciences, Umeå University, Umeå, Sweden;Institute of Clinical Biochemistry, The National Hospital, University of Oslo, Oslo, Norway;Norwegian College of Veterinary Medicine, Oslo, Norway;To whom correspondence should be addressed.;

关键词: remnants;    retinyl esters;    triglycerides;    fatty acids;    apolipoprotein B;   

DOI  :  

来源: DOAJ

【 摘 要 】

Mink homozygous for the mutation Pro214Leu in lipoprotein lipase (LPL) had only traces of LPL activity but amounts of LPL protein in their tissues similar to those of normal mink. In normal mink, lymph chylomicrons from rats given [3H]retinol (incorporated into retinyl esters, providing a core label) and [14C]oleic acid (incorporated mainly in triglycerides (TG)) were rapidly cleared from the circulation. In the homozygous mink, clearance was much retarded. The ratio of TG to core label in plasma did not decrease and much less [14C]oleic acid appeared in plasma. Still, half of the labeled material disappeared from the circulating blood within 30–40 min and the calculated total turnover of TG in the hypertriglyceridemic mink was almost as large as in normal mink. The core label was distributed to the same tissues in hypertriglyceridemic mink as in normal mink. Half to two-thirds of the cleared core label was in the liver. The large difference was that in the hypertriglyceridemic mink, TG label (about 40% of the total amount removed) followed the core label to the liver and there was no preferential uptake of TG over core label in adipose or muscle tissue. In normal mink, only small amounts of TG label (<10%) appeared in the liver, while most was in adipose and muscle tissues. Apolipoprotein B-48 dominated in the accumulated TG-rich lipoproteins in blood of hypertriglyceridemic mink, even in fasted animals.—Savonen, R., K. Nordstoga, B. Christophersen, A. Lindberg, Y. Shen, M. Hultin, T. Olivercrona, and G. Olivecrona. Chylomicron metabolism in an animal model for hyperlipoproteinemia type I. J. Lipid Res. 1999. 40: 1336–1346.

【 授权许可】

Unknown