MiRNA-34c Regulates Bovine Sertoli Cell Proliferation, Gene Expression, and Apoptosis by Targeting the AXL Gene

Animals

Ming Gao¹  Hao Sun¹  Zitong Bai¹  Runjun Yang¹  Lixin Xia¹  Xibi Fang¹  Haibin Yu²  Zhihui Zhao²  Ping Jiang² 

[1] College of Animal Science, Jilin University, Changchun 130062, China;College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China;

关键词: miR-34c;    Sertoli cells;    proliferation;    synthesis;    apoptosis;    AXL;   

DOI  :  10.3390/ani11082393

来源: DOAJ

【 摘 要 】

MicroRNAs (miRNAs) play significant roles in mammalian spermatogenesis. Sertoli cells can provide a stable microenvironment and nutritional factors for germ cells, thus playing a vital role in spermatogenesis. However, few studies elucidate the regulation of bovine testicular Sertoli cells by miRNAs. Here, we have reported that miRNA-34c (miR-34c) regulates proliferation, apoptosis, and relative transcripts abundance gene in bovine Sertoli cells. In bovine Sertoli cells, overexpression of miR-34c inhibited proliferation and relative abundance of gene transcripts while promoting apoptosis of Sertoli cells, and the effects were the opposite when miR-34c was knocked down. Receptor tyrosine kinase (AXL) was identified as a direct target gene of miR-34c in Sertoli cells, validated by analysis of the relative abundance of AXL transcript and dual-luciferase reporter assay. The relative abundance of the transcript of genes related to male reproduction in Sertoli cells was changed after the AXL gene was overexpressed, as demonstrated by the RT2 Profiler PCR Array results. In summary, miR-34c specifically regulated the AXL gene by targeting a sequence in the 3′-UTR, which could influence proliferation, apoptosis, and relative abundance of the transcript of male reproduction-related genes. Therefore, miR-34c could be considered an essential regulator in the process of bull spermatogenesis.

【 授权许可】

Unknown