Changes in Ultrastructure and Cytoskeletal Aspects ofHuman Normal and Osteoarthritic Chondrocytes Exposedto Interleukin-1β and Cyclical Hydrostatic Pressure

International Journal of Molecular Sciences

Antonella Fioravanti¹  Nicola Antonio Pascarelli¹  Sara Cheleschi¹  Elena Moretti²  Giulia Collodel² 

[1] Department of Medicine, Surgery and Neuroscience, Rheumatology Unit, University of Siena, Policlinico Le Scotte, Viale Bracci 1, Siena 53100, Italy;Department of Molecular and Developmental Medicine, University of Siena, Siena 53100, Italy;

关键词: cytoskeleton;    human chondrocytes;    hydrostatic pressure;    interleukin-1β;    osteoarthritis;    ultrastructure;   

DOI  :  10.3390/ijms161125936

来源: DOAJ

【 摘 要 】

The aim of this study was to examine the ultrastructure and cytoskeletal organization in human normal and Osteoarhritic (OA) chondrocytes, exposed tointerleukin-1β (IL-1β) and cyclic hydrostatic pressure (HP). Morphological examination by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed differences between normal and OA chondrocytes at the nuclear and cytoplasmic level. IL-1β (5 ng/mL) induced a decrease of the number of mitochondria and Golgi bodies and a significant increase on the percentage of cells rich in vacuolization and in marginated chromatin. Cyclical HP (1–5 MPa, 0.25 Hz, for 3 h) did not change the morphology of normal chondrocytes, but had a beneficial effect on OA chondrocytes increasing the number of organelles. Normal and OA cells subjected to IL-1β and HP recovered cytoplasmic ultrastructure. Immunofluorescence (IF) examination of normal chondrocytes showed an actin signal polarized on the apical sides of the cytoplasm, tubulin and vimentin uniformly distributed throughout cytoplasm and vinculin revealed a punctuated pattern under the plasma membrane. In OA chondrocytes, these proteins partially lost their organization. Stimulation with IL-1β caused, in both type of cells, modification in the cytoskeletal organization; HP counteracted the negative effects of IL-1β. Our results showed structural differences at nuclear, cytoplasmic and cytoskeletal level between normal and OA chondrocytes. IL-1β induced ultrastructural and cytoskeletal modifications, counteracted by a cyclical low HP.

【 授权许可】

Unknown