The Effects of Rapamycin on the Proliferation, Migration, and Apoptosis of Human Tracheal Fibroblasts (HTrF) and Human Tracheal Epithelial Cells (HTEpiC)

Journal of Clinical Medicine

Yan Liu¹  Juan Wang¹  Ting Wang¹  Jie Zhang¹  Jianhai Long¹  Xiaojian Qiu¹ 

[1] Department of Respiratory, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China;

关键词: restenosis;    airway stent;    drug-eluting stent;    human tracheal fibroblasts (HTrF);    human tracheal epithelial cells (HTEpiC);    rapamycin;   

DOI  :  10.3390/jcm11030608

来源: DOAJ

【 摘 要 】

Background: Restenosis after airway stenting needs to be addressed urgently. Rapamycin has been proven to inhibit restenosis elsewhere. This study aimed at observing its effects on the respiratory tract. Methods: CCK-8, wound healing, Transwell and apoptosis assays were performed to detect the effects of rapamycin on the survival, migration, and apoptosis, respectively, of human tracheal fibroblasts (HTrF) and human tracheal epithelial cells (HTEpiC). Results: The effective concentrations of paclitaxel, mitomycin C and rapamycin on HTrF were 10⁻⁷–10⁻⁴ mol/L, 10⁻⁶–10⁻⁴ mol/L, and 10⁻⁵–10⁻⁴ mol/L, respectively. At the effective concentrations, the inhibition rates of paclitaxel on HTEpiC were (43.03 ± 1.12)%, (49.49 ± 0.86)%, (55.22 ± 1.43)%, and (93.19 ± 0.45)%; the inhibition rates of mitomycin C on HTEpiC were (88.11 ± 0.69)%, (93.82 ± 0.96)%, and (94.94 ± 0.54)%; the inhibition rates of rapamycin on HTEpiC were (10.19 ± 0.35)% and (94.55 ± 0.71)%. At the concentration of (1–4) × 10⁻⁵ mol/L, the inhibition rate of rapamycin on HTrF was more than 50%, and that on HTEpiC was less than 20% (p < 0.05). Conclusions: Compared to paclitaxel and mitomycin C, rapamycin had the least effect on HTEpiC while effectively inhibiting HTrF. The optimum concentration range was (1–4) × 10⁻⁵ mol/L.

【 授权许可】

Unknown