Optimization of Ultrasonic-assisted Extraction of Fatty Acids in Seeds of Brucea Javanica (L.) Merr. from Different Sources and Simultaneous Analysis Using High-Performance Liquid Chromatography with Charged Aerosol Detection

Molecules

Yiqiong Pu¹  Zhuona Wu¹  Ling Li¹  Tong Zhang¹  Yue Zhang¹  Bing Wang¹  Ning Li²  Xitong Zhang³ 

[1] Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Pudong New District, Shanghai 201203, China;Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, No. 1200, Cailun Road, Pudong New District, Shanghai 201203, China;Shanghai Xiangshan Hospital of Traditional Chinese Medicine, No. 528, Middle Fuxing Road, Huangpu District, Shanghai 200020, China;

关键词: charged aerosol detector;    chromatographic fingerprint;    fatty acids;    high-performance liquid chromatography;    response surface methodology;   

DOI  :  10.3390/molecules22060931

来源: DOAJ

【 摘 要 】

Our research aimed to optimize the oil extraction process and determine the fatty acids in Brucea javanica (L.) Merr. seeds. The extraction technology was optimized using response surface methodology. A Box-Behnken design was employed to investigate the effects of three independent variables on an ultrasonic-assisted extraction technique, namely, sonication time (X1: 20–40 min), liquid–solid ratio (X2: 16:1 mL/g–24:1 mL/g), and ethanol concentration (X3: 90%–100%). The optimum conditions of sonication time, liquid–solid ratio, and ethanol concentration were 40 min, 24:1 mL/g, and 100%, respectively. The content of fatty acids and the oil yield were 14.64 mg/g and 16.87%, respectively, which match well with the predicted models. The optimum number of extraction times was eventually identified as two. A new rapid method for the qualitative and quantitative analysis of the fatty acids of B. javanica (L.) Merr. seed oil using HPLC with a charged aerosol detector was described. The fatty acid contents of 14 batches of B. javanica (L.) Merr. seed oil were determined, and the relevance and difference were analyzed by fingerprint analysis. The fingerprint has five common peaks, and the similarity was greater than 0.991. HPLC analysis represents a specialized and rational approach for the quality identification and comprehensive evaluation of B. javanica (L.) Merr. seed oils.

【 授权许可】

Unknown