Vibrio deploys type 2 secreted lipase to esterify cholesterol with host fatty acids and mediate cell egress

eLife

Kim Orth¹  Jeffrey G McDonald²  Marcela de Souza Santos³  Krzysztof Pawlowski⁴  Jen Liou⁴  Gonçalo Vale⁵  Wan-Ru Lee⁶  Ann Ray⁷  Suneeta Chimalapati⁷  Matthew A Mitsche⁷  Alexander E Lafrance⁷  Giomar Rivera-Cancel⁸ 

[1] Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, United States;Faculty of Agriculture and Biology, Warsaw University of Life Sciences, Warsaw, Poland;Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, United States;Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, United States;Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, United States;Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, United States;Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, United States;Department of Physiology, University of Texas Southwestern Medical Center, Dallas, United States;

关键词: T3SS;    vibrio parahaemolyticus;    egress;    invasion;    cholesterol homeostasis;    VPA0226;   

DOI  :  10.7554/eLife.58057

来源: DOAJ

【 摘 要 】

Pathogens find diverse niches for survival including inside a host cell where replication occurs in a relatively protective environment. Vibrio parahaemolyticus is a facultative intracellular pathogen that uses its type 3 secretion system 2 (T3SS2) to invade and replicate inside host cells. Analysis of the T3SS2 pathogenicity island encoding the T3SS2 appeared to lack a mechanism for egress of this bacterium from the invaded host cell. Using a combination of molecular tools, we found that VPA0226, a constitutively secreted lipase, is required for escape of V. parahaemolyticus from the host cells. This lipase must be delivered into the host cytoplasm where it preferentially uses fatty acids associated with innate immune response to esterify cholesterol, weakening the plasma membrane and allowing egress of the bacteria. This study reveals the resourcefulness of microbes and the interplay between virulence systems and host cell resources to evolve an ingenious scheme for survival and escape.

【 授权许可】

Unknown