Hair Histology and Glycosaminoglycans Distribution Probed by Infrared Spectral Imaging: Focus on Heparan Sulfate Proteoglycan and Glypican-1 during Hair Growth Cycle

Biomolecules

Vincent Bardey¹  Solène Mine¹  Nicolas Berthélémy¹  Louis Danoux¹  Christine Jeanmaire¹  Laurent Ramont²  Stéphane Brézillon²  Charlie Colin-Pierre²  GaneshD Sockalingum³  Valérie Untereiner⁴ 

[1] BASF Beauty Care Solutions France SAS, 54425 Pulnoy, France;Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 51097 Reims, France;Université de Reims Champagne-Ardenne, BioSpecT EA7506, UFR de Pharmacie, 51097 Reims, France;Université de Reims Champagne-Ardenne, PICT, 51097 Reims, France;

关键词: hair follicle growth;    glycosaminoglycans;    infrared spectral imaging;    k-means clustering;    immunohistochemistry;   

DOI  :  10.3390/biom11020192

来源: DOAJ

【 摘 要 】

The expression of glypicans in different hair follicle (HF) compartments and their potential roles during hair shaft growth are still poorly understood. Heparan sulfate proteoglycan (HSPG) distribution in HFs is classically investigated by conventional histology, biochemical analysis, and immunohistochemistry. In this report, a novel approach is proposed to assess hair histology and HSPG distribution changes in HFs at different phases of the hair growth cycle using infrared spectral imaging (IRSI). The distribution of HSPGs in HFs was probed by IRSI using the absorption region relevant to sulfation as a spectral marker. The findings were supported by Western immunoblotting and immunohistochemistry assays focusing on the glypican-1 expression and distribution in HFs. This study demonstrates the capacity of IRSI to identify the different HF tissue structures and to highlight protein, proteoglycan (PG), glycosaminoglycan (GAG), and sulfated GAG distribution in these structures. The comparison between anagen, catagen, and telogen phases shows the qualitative and/or quantitative evolution of GAGs as supported by Western immunoblotting. Thus, IRSI can simultaneously reveal the location of proteins, PGs, GAGs, and sulfated GAGs in HFs in a reagent- and label-free manner. From a dermatological point of view, IRSI shows its potential as a promising technique to study alopecia.

【 授权许可】

Unknown