Butein is a novel anti-adipogenic compound[S]

Journal of Lipid Research

Jeong-Soo Lee¹  Young Min Lee²  Ki-Moon Park³  No-Joon Song³  Hyang-Jin Yoon³  Woo-Seok Jang³  So-Ra Jung³  Cho-Rong Seo³  Dae-Hyuk Kweon⁴  Joung-Woo Hong⁵  Ki Hyun Kim⁶  Kang Ro Lee⁶  Kye Won Park⁷ 

[1] Aging Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea;Department of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-853, Republic of Korea;Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Republic of Korea;Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea;Graduate School of East-West Medical Science, Kyung Hee University, Yongin 446-701, Republic of Korea;Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea;To whom correspondence should be addressed [e-mail];

关键词: adipocyte differentiation;    peroxisome proliferator-activated receptor γ;    butein;    obesity;    Rhus verniciflua Stokes;    C3H10T1/2;   

DOI  :  

来源: DOAJ

【 摘 要 】

Rhus verniciflua Stokes (RVS) has been used as a traditional herbal medicine for its various biological activities including anti-adipogenic effects. Activity-guided separation led to the identification of the anti-adipogenic functions of butein. Butein, a novel anti-adipogenic compound, robustly suppressed lipid accumulation and inhibited expression of adipogenic markers. Molecular studies showed that activated transforming growth factor-β (TGF-β) and suppressed signal transducer and activator of transcription 3 (STAT3) signaling pathways were mediated by butein. Analysis of the temporal expression profiles suggests that TGF-β signaling precedes the STAT3 in the butein-mediated anti-adipogenic cascade. Small interfering RNA-mediated silencing of STAT3 or SMAD2/3 blunted the inhibitory effects of butein on adipogenesis indicating that an interaction between two signaling pathways is required for the action of butein. Upon butein treatments, stimulation of TGF-β signaling was still preserved in STAT3 silenced cells, whereas regulation of STAT3 signaling by butein was significantly impaired in SMAD2/3 silenced cells, further showing that TGF-β acts upstream of STAT3 in the butein-mediated anti-adipogenesis. Taken together, the present study shows that butein, a novel anti-adipogenic compound from RVS, inhibits adipocyte differentiation through the TGF-β pathway followed by STAT3 and peroxisome proliferator-activated receptor γ signaling, further implicating potential roles of butein in TGF-β- and STAT3-dysregulated diseases.

【 授权许可】

Unknown