Long-term survival of cultivated oral mucosal epithelial cells in human cornea: generating cell sheets using an animal product-free culture protocol

Stem Cell Research & Therapy

Chi-Chin Sun¹  An-Shine Chao²  Ming-Tse Kuo³  Shiang-Fu Huang⁴  Jui-Yang Lai⁵  Kevin Sheng-Kai Ma⁶  David Hui-Kang Ma⁶  Hung-Chi Chen⁶  Yi-Jen Hsueh⁶  Yueh-Ju Tsai⁶ 

[1] Department of Medicine, School of Medicine, College of Medicine, Chang Gung University;Department of Obstetrics and Gynecology, New Taipei Municipal Tucheng Hospital;Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine;Department of Otolaryngology, Chang Gung Memorial Hospital;Graduate Institute of Biomedical Engineering, Chang Gung University;Limbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital;

关键词: COMET;    Collagenase;    Oral mucosa;    Amniotic membrane;    Microsphere;   

DOI  :  10.1186/s13287-021-02564-7

来源: DOAJ

【 摘 要 】

Abstract Previously, we reported a collagenase-based, animal product-free protocol for cultivated oral mucosal epithelial cell sheets for transplantation (COMET). Here, we reported the long-term outcomes of first 2 clinical cases. A 27-year-old man suffered from thermal burn, which resulted in symblepharon of lower fornix OD. COMET was performed, and the cornea remained clear with few peripheral NV and no more symblepharon 34 months postoperatively. Another 42-year-old man suffered from severe alkaline burn OD. He underwent COMET, followed by corneal transplantation half a year later. A biopsy taken two years after COMET showed stratified epithelium positive for keratin 4, 13, and 3 in the suprabasal layer. Staining for p63 and p75NTR was both positive in the basal layer. The graft remained clear up to post-OP 4 years. Our study confirmed the long-term survival of the transplanted OMECs, suggesting that collagenase-based spheroidal suspension culture is a promising technique for COMET. Trial registration ClinicalTrials.gov, ClinicalTrials.gov ID: NCT03943797 Registered 9 May 2019-Retrospectively registered, https://clinicaltrials.gov/ct2/show/NCT03943797 .

【 授权许可】

Unknown